Polo-Like Kinase (phospho T210) polyclonal antibody
产品名称： Polo-Like Kinase (phospho T210) polyclonal antibody
英文名称： Polo-Like Kinase (phospho T210) polyclonal antibody
产品编号： PAB9614
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Polo-Like Kinase.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding T210 of human and rat Polo-Like Kinase.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 66
- Reactivity:
- Bovine, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
- Form:
- Liquid
- Purification:
- Affinity purification
- Storage Buffer:
- In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1.
van de Weerdt BC, van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH.Mol Cell Biol. 2005 Mar;25(5):2031-44.
- 2.
- Polo-like kinases (Plks) and cancer.
Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I.Oncogene. 2005 Jan 10;24(2):287-91.
- 3.
- Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis.
van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH.J Biol Chem. 2004 Aug 27;279(35):36841-54. Epub 2004 Jun 21.
- Applications
- Western Blot (Tissue lysate)
- Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k Polo-Like Kinase protein phosphorylated at Thr210 (control). The phosphospecificity of this abeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Polo-Like Kinase (phospho T210) polyclonal antibody (Cat # PAB9614). The mmunolabeling is completely eliminated by treatment with lambda-Ptase.